Aberrant overexpression of 53BP2 mRNA in lung cancer cell lines

The p53-binding protein 2 (53BP2) was identified as a binding protein to a turner suppressor p53. We examined the genetic aberrations of 53BP2 gene in various human cancer cell lines. Although no gross genomic alteration or m utation of 53BP2 gene mas observed, 53BP2 mRNA levels were highly variable. There was no association between the 53BP2 mRNA level and the p53 status. When we examined sensitivities of these cell lines to DNA-damaging agents i ncluding UV irradiation, X-ray irradiation and cis-diamine-dichloroplatinum (CDDP), we found that higher 53BP2 mRNA expression nas correlated with the sensitivity to these agents. (C) 2000 Federation of European Biochemical S ocieties.