Tension-voltage relationship in membrane fusion and its implication in exocytosis

In this study, new methods are used to control cellular membrane tension to evaluate the role it plays in electrofusion. The data show that membrane t ension present during the application of an electric field facilitates elec tro-induced membrane fusion, No enhancement was detected if the strain was applied after the pulse. Analysis of the electromechanical process of fusio n revealed a synergy between the two kinds of constraints in the membrane f usion, Both mechanical and electrical constraints apparently play a kev rol e in membrane fusion between the granule membrane and the plasma membrane, i.e. the exocytosis process. (C) 2000 Federation of European Biochemical So cieties.