Visualization of carbohydrate-binding molecules expressed by myelomono- and erythropoietic cells derived from human bone marrow: An immunoenzymatic double-staining study

Citation
Se. Baldus et al., Visualization of carbohydrate-binding molecules expressed by myelomono- and erythropoietic cells derived from human bone marrow: An immunoenzymatic double-staining study, HISTOCHEM J, 32(1), 2000, pp. 27-32
Citations number
52
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
HISTOCHEMICAL JOURNAL
ISSN journal
00182214 → ACNP
Volume
32
Issue
1
Year of publication
2000
Pages
27 - 32
Database
ISI
SICI code
0018-2214(200001)32:1<27:VOCMEB>2.0.ZU;2-U
Abstract
Interactions between human haematopoietic and bone marrow stromal cells are governed by complex carbohydrate-mediated adhesion processes. In order to evaluate corresponding carbohydrate-binding sites on human myelo- and eryth ropoietic cells which were able to react with mono- or oligosaccharides, we established an immunocytochemical double-staining assay. In a first step, cell lineages were visualized using Lewis(x) (CD15) or glycophorin C-specif ic monoclonal antibodies. The second step included polyacrylamide-conjugate d carbohydrate structures. According to our results, the carbohydrate-bindi ng potential of granulopoietic cells increased during the process of matura tion, contrasting a reduction of carbohydrate-binding sites on erythroid pr ecursor cells during differentiation. With respect to previous in vitro stu dies, these findings shed some light on certain aspects of bone marrow homi ng as well as on the trafficking of mature cellular elements into circulati on. It is tempting to speculate that carbohydrate-mediated adhesion mechani sms may be involved in the various functional defects of progenitor cells i n chronic myelogenic leukaemia, especially regarding their complex interact ions with the marrow microenvironment.