S. Mcbride et al., Characterisation of lectin binding patterns of mouse bronchiolar and rat alveolar epithelial cells in culture, HISTOCHEM J, 32(1), 2000, pp. 33-40
Lung epithelial cell differentiation pathways remain unclear. This is due i
n part to the plasticity of these cells and the lack of markers which accur
ately reflect their differentiation status. The aim of this study was to de
termine if lectin binding properties are useful determinants of functional
differentiation status in vitro. Mouse Clara cells were cultured for 5 days
. During this time, no alteration in differentiation was evident by electro
n microscopy. No significant alteration in binding reactivity of Bauhinia p
urpurea (BPA), Maclura pomifera (MPA), Concanavalin A, Wheat germ or Helix
pomatia lectins occurred in cultures compared with Clara cells in mouse lun
g tissue. In contrast, nitrotetrazolium blue reductase activity and CC10 ex
pression declined in culture. Rat type II cells were cultured for 8 days. B
etween days 0 and 4, the number of type II cells identified by electron mic
roscopy was constant at 70-80%, decreasing to 8% by day 6. In contrast, by
day 4, only 42% cells retained alkaline phosphatase activity. BPA and MPA r
eactivity was altered at day 0 and day 4 respectively, compared with cells
in situ. Therefore, the reactivity of lectins analysed here does not reflec
t functional differentiation status of cultured mouse Clara cells. However,
BPA and MPA reactivity may be a sensitive indicator of alterations in rat
type II cell differentiation in vitro.