In-vitro assessment of crossbred buck fertility

A study was conducted on 10 crossbred bucks (aging 26 to 50 months) to eval uate their fertility status on the basis of semen quality and hypo-osmotic swelling test (HOS), cervical mucus penetration test (CMPT) and resistance to 1% NaCl solution during 3 seasons (hot-dry, hot-humid and cold-humid). C ollections (150) were made and the quality of semen was evaluated using sta ndard procedures. The analysis of semen revealed that, resistance value was significantly (P<0.01) affected by season. Season, buck and season x buck interaction significantly (P<0.01) influenced HOS-reacted and not-reacted s permatozoa. Higher resistance value to 1% NaCl solution was obtained during cold-humid season. pH of the cervical mucus had significant (P<0.01) effec t on the in vitro penetration rate of goat spermatozoa. HOS test can be use d for predicting fertilizing ability of spermatozoa and being simple and ra pid, it can be used as a routine assessment technique in laboratories. Ther e is a need to have some criteria for selection of bucks on the basis of se men quality and in vitro assessment of fertility and fertility rate.