A gl-year-old woman was referred to our hospital for refractory thrombocyto
penia (3 x 10(3)/mu) and massive melena. Bone marrow aspiration revealed no
rmal cellularity and increased megakaryocytes (250/mu). An abdominal comput
erized axial tomography scan showed isodensity masses on both adrenal gland
s. 67Ga-scintigraphy exhibited strong uptake into the bilateral adrenal tum
or and mediastinal region. IgM-type antibody against platelet glycoprotein
Ib (GpIb) was detected in the patient's serum. A needle biopsy of the right
adrenal tumor was performed, and histology was non-Hodgkin's lymphoma (NHL
) diffuse large B-cell type. Following the diagnosis of autoimmune thromboc
ytopenia associated with lymphoma. administration of corticosteroid (predon
isolone 60 mg/day) and high-dose intravenous globulin (15 g/day x 4 days) w
as carried out, but neither was effective in normalizing the thrombocytopen
ia. Immunosuppressive therapy (cyclophosphamide 500 mg and 1 mg of vincrist
ine) markedly restored the platelet counts to 7.2 x 10(4)/mu and ceased the
melena; furthermore, the size of adrenal tumors decreased by more than 60%
after therapy. We cultured the lymphoma cells drawn by needle biopsy with
IL-6 in vitro and found that the lymphoma cells produced IgM-type antiplate
let antibodies against platelet GpIb in the culture supernatant. Thus this
is a rare case of NHL in which the production of antiplatelet antibody from
lymphoma cells was confirmed in vitro. (C) 1999 The Japanese Society of He
matology.