Detection of integrins in cataract lens epithelial cells

Purpose: To detect the expression of integrin subunits in lens epithelial c ells (LECs) of human cataracts. Setting: Research Laboratory, International Intraocular Implant Training Ce ntre Tianjin Medical University, China. Methods: The circular sections of the anterior capsules with attached LECs were obtained during cataract surgery from 100 patients. The LECs were stai ned with an avidin-biotin-complex immunohistochemical technique using 5 mon oclonal antibodies specific for alpha subunits 2, 3, 5 and beta subunits 1, 2. Results: All integrin subunits studied were found to varying degrees in spe cimens. The positive percentages were 70%, 65%, 75%, 70%, and 80%, respecti vely. Conclusion: Integrin subunits were present in LECs of human cataracts. Thes e molecules may serve in the adhesion of LECs to the lens capsule and play a role in cell-posterior capsule interaction after cataract surgery. (C) 20 00 ASCRS and ESCRS.