Adsorption/partition model of liquid chromatography for chemically bonded stationary phases of the aliphatic cyano, reversed-phase C-8 and reversed-phase C-18 types

A novel approach was introduced to modeling solute retention in the liquid chromatography systems, employing silica-based aliphatic chemically bonded stationary phases of the cyano, reversed-phase C-g and reversed-phase C-18 types, and the mixed binary eluents most frequently used in the reversed-ph ase and normal-phase chromatography modes (i.e. using the methanol-water an d the 2-propanol-n-hexane liquid mixtures, respectively). This approach tak es notice of the mixed (adsorption/partition) mechanism of solute retention , in which both, the adsorptive and the dispersive forces contribute to the overall energetics of this process. Performance of our new model was compa red with that of the widely recognized and on a routine basis applied Schoe nmakers approach, and it was found out that both models perform with a prac tically equal and outstanding accuracy. (C) 2000 Elsevier Science B.V. All rights reserved.