Repeatability and reproducibility of retention data and band profiles on reversed-phase liquid chromatography columns - IV. Results obtained with tuna C-18 (2) columns

The reproducibility of retention data and band profile characteristics was investigated for a series of columns packed with Luna C-18 (2), a silica-ba sed, reversed-phase adsorbent. High precision data were obtained and statis tically compared among five columns from the same batch (column-to-column r eproducibility) and nine columns from as many different batches (batch-to-b atch reproducibility). These data were acquired under five different sets o f chromatographic conditions, for a group of 30 neutral, acidic, and basic compounds selected as probes following an experimental protocol previously described. This work is part of a study on the precision of chromatographic analyses. Its purpose is to illustrate the contribution of the stationary phase reproducibility to this precision. (C) 2000 Elsevier Science B.V. All rights reserved.