Control of column temperature in reversed-phase liquid chromatography

When separations by reversed-phase liquid chromatography (RP-LC) are carrie d out at temperatures other than ambient, resulting retention times and ban dwidths can depend on the equipment used. As a result, an RP-LC separation that is adequate when carried out on one LC system may prove inadequate whe n the separation is repeated on a second system. In the present study, vari ous temperature-related problems which can result in a failure of method tr ansfer for non-ambient RP-LC methods were examined. Means for correcting fo r such effects and thereby ensuring method transferability are described. ( C) 2000 Elsevier Science B.V. All rights reserved.