Migration time correction for the analysis of derivatized amino acids and oligosaccharides by micellar capillary electrochromatography

Migration-time reproducibility is essential in the use of capillary electro phoresis to identify components in mixtures. Two methods based on the migra tion time of either one or two reference markers are proposed for improving migration time reproducibility. These methods were evaluated to determine the migration time reproducibility for phenylthiohydantoin-amino acids, flu orescein thiohydantoin-amino acids, and tetramethylrhodamine labeled oligos accharides. In the best case, the relative standard deviation of the migrat ion time was reduced from >3% without correction to <0.04% with the two-mar ker correction. (C) 2000 Elsevier Science B.V. All rights reserved.