Improved endothelialization of vascular grafts by local release of growth factor from heparinized collagen matrices

Endothelial cell seeding, a promising method to improve the performance of small-diameter vascular grafts, requires a suitable substrate, e.g. crossli nked collagen. In addition, the growth of seeded endothelial cells can be i mproved by local release of a heparin-binding protein, basic fibroblast gro wth factor (bFGF). In this study, the influence of immobilization of hepari n to collagen, crosslinked using N-(3-dimethylaminopropyl)-N'-ethylcarbodii mide (EDC) in combination with N-hydroxysuccinimide (NHS), on the binding a nd release of bFGF was determined. Heparin was immobilized also using EDC a nd NHS. Furthermore, the effects of the release of bFGF from (heparinized) EDC/NHS-crosslinked collagen on the proliferation of seeded endothelial cel ls was studied in vitro. Immobilization of increasing amounts of heparin to EDC/NHS-crosslinked collagen (containing 14 free epsilon-amino groups per 1000 amino acid residues, E/N14C) resulted in binding of increasing amounts of bFGF to the material. Maximal bFGF binding was observed for E/N14C cont aining 20-30 mg heparin immobilized per gram of collagen which was obtained using a molar ratio of EDC to heparin-carboxylic acid groups of 0.4 for he parin immobilization (E/N14C-H(0.4)). Up to concentrations of 320 ng bFGF/m l, 10% of the added bFGF bound to E/N14C, while binding of bFGF to E/N14C-H (0.4) was 22%. The initial release rate of bFGF bound to E/N14C was much hi gher compared to bFGF bound to E/N14C-H(0.4): respectively, 30 vs. 2% in th e first 6 h. After 10 days, the bFGF release from E/N14C and E/N14C-H(0.4) amounted to 83 vs. 42%, respectively. Binding of increasing amounts of bFGF resulted in increased growth of human umbilical vein endothelial cells (HU VECs) seeded on both E/N14C and E/N14C-H(0.4). Nevertheless, after 6 and 10 days of proliferation cell numbers on E/N14C-H(0.4) where higher than cell numbers on E/N14C, irrespective of the bFGF concentration used for loading of the matrix. It is concluded that heparinized, EDC/NHS-crosslinked colla gen is a good synthetic vascular graft coating for in vivo endothelial cell seeding. (C) 2000 Elsevier Science B.V. All rights reserved.