Simultaneous high performance liquid chromatographic analysis of acetaminophen, salicylamide, phenyltoloxamine, and related products

A stability indicating high performance liquid chromatography method has be en developed for simultaneous determination of acetaminophen, salicylamide and phenyltoloxamine. The reversed-phase method utilizes UV detection at 22 0 nm and a C8 column. This paper presents the data to support linearity, pr ecision, specificity, and robustness of the method. The known potential deg radation products of acetaminophen, p-aminophenol, p-nitrophenol, precursor impurity p-hydroxyacetophenone, the potential degradation product of salic ylamide, salicylic acid, and precursor impurity ethylsalicylate were separa ted for quantitation simultaneous with parent compounds. Quantification was achieved by peak area and external standard method. This method can be emp loyed in determining stability, assay, content uniformity, and dissolution of the combination in pharmaceutical dosage forms.