We studied microhabitat use by Proechimys semispinous (Central American spi
ny rat) by Livetrapping along transects in 5 forested sites in central Pana
ma. Microhabitat was quantified by measuring 14 variables at each sampling
station. Relative abundance of P. semispinosus was 1.86 individuals/100 sta
tion-nights, which was typical of abundances of this species and other memb
ers of the genus. Spiny rats were distributed throughout sampled microhabit
at space defined by factor analysis of 14 variables. We used logistic regre
ssion analysis to relate presence-absence at trap stations to microhabitat
structure. Variables describing disturbed and younger forest were strong pr
edictors of presence at trap stations. Associations of P. semispinosus with
younger forest (smaller trees and lianas and lower canopies) and treefall
gaps within older forest may have implications for regeneration of local fo
rest patches through the activities of these rodents as seed predators and
dispersers of seeds and arbuscular mycorrhizal fungi spares.