Development of glutamatergic synaptic activity in cultured spinal neurons

The development of glutamatergic synapses involves a sequence of events tha t are still not well understood. We have studied the time course of the dev elopment of glutamatergic synapses in cultured spinal neurons by characteri zing spontaneous synaptic currents recorded from cells maintained in vitro for different times. At short times in culture (2 days in vitro; DIV2), spo ntaneous synaptic activity consisted almost solely of N-methyl-D-aspartate (NMDA) receptor (NMDAR) openings. In contrast, older neurons (DIV5 to DIV8) displayed clear alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid ( AMPA) receptor (AMPAR)-mediated synaptic currents, while the NMDAR-mediated activity remained small. Between 8 and 14 days in vitro there was a large increase in the density of synaptically activated NMDARs, although there wa s no significant increase in the density of the NMDAR-mediated current acti vated by exogenous glutamate. The results indicate that there is a switch i n NMDAR targeting from somatic to synaptic regions during the course of the second in vitro week. Finally, our results support the conclusion that the spontaneous synaptic activity displayed in culture depends on ongoing NMDA R-mediated activity, even when the expression of synaptic NMDARs is low.