Effects of intracolonic opioid receptor agonists on polymodal pelvic nerveafferent fibers in the rat

Effects of intracolonic opioid receptor agonists on polymodal pelvic nerve afferent fibers in the rat. J. Neurophysiol. 83: 963-970, 2000. We studied the effects of intra colonic administration of opioid receptor agonists (OR As) on responses of pelvic nerve efferent fibers to colorectal distension ( CRD) and heat. Single-fiber recordings were made from the decentralized S1 dorsal rootlet in the rat. An similar to 7-cm length of descending colon wa s isolated in situ to permit intracolonic perfusion with Krebs solution, wh ich, when the outflow was clamped, was used to distend the colon. Responses to noxious CRD (40 mmHg, 30 s) were tested after intracolonic instillation of mu-, delta- or kappa-ORAs. Intracolonic administration of the kappa-ORA s EMD 61,753 (n = 5/12) and U62,066 (n = 8/11), but not either the mu-ORA f entanyl or the delta-ORA SNC-80, concentration-dependently inhibited respon ses of afferent fibers. For fibers unaffected by intracolonic administratio n of EMD 61,753 or U62,066, intra-arterial administration of kappa-ORAs was effective. Forty-one of 54 mechanosensitive fibers also responded to intra colonic instillation of heated Krebs solution (50 degrees C). Intra-arteria l injection of fentanyl or SNC-80 did not attenuate responses to heat. Eith er intracolonic or intra-arterial administration of EMD 61,753 or U62,066, however, inhibited afferent fiber responses to heat. These results document that mechanical and thermal sensitivity of polymodal pelvic nerve afferent fibers innervating the rat colon can be inhibited peripherally by intracol onic instillation of kappa-ORAs.