Electrochemical studies of the interaction of adriamycin to DNA

An electrochemical investigation of the interaction of adriamycin (DXH) wit h DNA on a Hg electrode is reported. In weakly acidic media of pH 4.0-7.0, the addition of DNA to DXH solution results in the decrease of redox peak c urrents.:In the presence of DNA, no new peak appears and the standard rats constant k(s) is not significantly changed. The binding of DXH to DNA by el ectrostatic attraction and intercalation forms a kind of supramolecular com plex DXH-DNA, which is electrochemically non-active. The equilibrium consta nt for the complex is calculated. The decrease in peak current is proportio nal to DNA concentration and can be used to determine DNA concentration. (C ) 2000 Elsevier Science B.V. All rights reserved.