Detection of Pectinatus spp. by PCR using 16S-23S rDNA spacer regions

Authors
Motoyama, Y Ogata, T
Citation
Y. Motoyama et T. Ogata, Detection of Pectinatus spp. by PCR using 16S-23S rDNA spacer regions, J AM SOC BR, 58(1), 2000, pp. 4-7
Citations number
10
Categorie Soggetti
Food Science/Nutrition
Journal title
JOURNAL OF THE AMERICAN SOCIETY OF BREWING CHEMISTS
ISSN journal
03610470 → ACNP
Volume
58
Issue
1
Year of publication
2000
Pages
4 - 7
Database
ISI
SICI code
0361-0470(2000)58:1<4:DOPSBP>2.0.ZU;2-8
Abstract
A polymerase chain reaction (PCR) method for the detection of Pectinatus ce revisiiphilus and P. frisingensis was developed. The 16S rRNA gene (rDNA) a nd the spacer regions between the 16S rDNA and the 23S rDNA genes were used for the PCR reaction. The species-specific sequences in the spacer region between the 16S rDNA and the 23S rDNA genes were selected for use as PCR pr imers. The method developed in this study was rapid and sensitive. Furtherm ore, this method allowed identification at the species level, even between very closely related species, such as P. cerevisiiphilus and P. frisingensi s.