Sweet potato (Ipornoea batatas L) tissue, when cooked at 70 degrees C to ac
tivate P-amylase and break down starch, takes on a distinctive firm, brittl
e texture and does not show the cell separation that occurs in, for example
, cooked potato (Solanum tuberosum L). Similar cooking conditions increase
firmness in other plants by activating pectin methyl esterase which de-este
rifies pectic polysaccharides and protects them from thermal depolymerisati
on. We therefore isolated cell walls from both potatoes and sweet potatoes
cooked at 70 degrees C and 100 degrees C and determined the remaining degre
e of methyl esterification of their pectins. Pectins from both species were
demethylated to a similar extent at: 70 degrees C and 100 degrees C. Since
cooking sweet potato at 100 degrees C induced cell separation and softenin
g, it is concluded that P-amylase is rapidly inactivated at that temperatur
e and swollen starch distends and separates the cells, whereas the firm tex
ture obtained by cooking that species at 70 degrees C is not the result of
pectin demethylation but is caused by the breakdown of starch to oligomers
that can escape from the cell, (C) 2000 Society of Chemical Industry.