Characterization of polyisoprene by temperature gradient interaction chromatography

Citation
W. Lee et al., Characterization of polyisoprene by temperature gradient interaction chromatography, MACRO CH P, 201(3), 2000, pp. 320-325
Citations number
23
Categorie Soggetti
Organic Chemistry/Polymer Science
Journal title
MACROMOLECULAR CHEMISTRY AND PHYSICS
ISSN journal
10221352 → ACNP
Volume
201
Issue
3
Year of publication
2000
Pages
320 - 325
Database
ISI
SICI code
1022-1352(200002)201:3<320:COPBTG>2.0.ZU;2-F
Abstract
Temperature gradient interaction chromatography (TGIC) analysis of polyisop rene (PI) is reported. PI can be fractionated in terms of molecular weight with high resolution by TGIC using a C18 bonded silica column as the statio nary phase and a mixture of CH2Cl2/CH3CN (80/20, v/v) as the mobile phase. The temperature was increased during the elution from 5 degrees C to 34 deg rees C to optimize the fractionation of high molecular weight PI. The relat ively poor resolution in the low molecular weight range was improved by cha nging the eluent composition. For example, all the oligomeric species from the unimer to the 29mer of a low molecular weight PI (average mol. wt.: -90 0) were fully resolved with the mixed eluent of CH2Cl2/CH3CN (50/50, v/v) a nd a linear temperature program from 5 degrees C to 40 degrees C. In additi on, simultaneous size exclusion chromatography and TGIC fractionation of PI /polystyrene, PI/poly(methylmethacrylate) mixtures are also reported.