Synthesis of hyaluronan of distinctly different chain length is regulated by differential expression of Xhas1 and 2 during early development of Xenopus laevis

Authors
Koprunner, M Mullegger, J Lepperdinger, G
Citation
M. Koprunner et al., Synthesis of hyaluronan of distinctly different chain length is regulated by differential expression of Xhas1 and 2 during early development of Xenopus laevis, MECH DEVEL, 90(2), 2000, pp. 275-278
Citations number
14
Categorie Soggetti
Cell & Developmental Biology
Journal title
MECHANISMS OF DEVELOPMENT
ISSN journal
09254773 → ACNP
Volume
90
Issue
2
Year of publication
2000
Pages
275 - 278
Database
ISI
SICI code
0925-4773(200002)90:2<275:SOHODD>2.0.ZU;2-5
Abstract
The localization of hyaluronan has been determined in tailbud stage embryos of Xenopus laevis using a neurocan-alkaline phosphatase fusion protein. Th is polysaccharide was located between the germ layers and enriched in mesen chyme, the lumen of the neural tube, the embryonic gut, the hepatic cavity and the heart. A full-length cDNA for a hyaluronan synthase, Xhas2 has been cloned, The expression pattern of Xhas1 and 2 is closely similar to the di stribution of hyaluronan in the embryo. Xhas1 produces hyaluronan with a mo lecular mass of around 40-200 kDa, while the product formed by Xhas2 has a molecular mass above 1 million Da. (C) 2000 Elsevier Science Ireland Ltd. A U rights reserved.