The feasibility of PCR-based diagnosis of Prader-Willi and Angelman syndromes using restriction analysis after bisulfite modification of genomic DNA

Citation
M. Velinov et al., The feasibility of PCR-based diagnosis of Prader-Willi and Angelman syndromes using restriction analysis after bisulfite modification of genomic DNA, MOL GEN MET, 69(1), 2000, pp. 81-83
Citations number
7
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MOLECULAR GENETICS AND METABOLISM
ISSN journal
10967192 → ACNP
Volume
69
Issue
1
Year of publication
2000
Pages
81 - 83
Database
ISI
SICI code
1096-7192(200001)69:1<81:TFOPDO>2.0.ZU;2-9
Abstract
We have developed a novel PCR-based method for studying DNA methylation in the proximal region of 15q, using restriction analysis after bisulfite trea tment of genomic DNA. This protocol can be used for the diagnosis of Prader -Willi and Angelman syndromes. Unlike the recently reported methylation-spe cific PCR protocol, our method avoids the use of multiplex amplification, t hus overcoming the need to adjust relative primer amounts and the risk of o btaining false-negative results. (C) 2000 Academic Press.