Cladistic analysis was performed on partial sequences from the nuclear-enco
ded small subunit ribosomal DNA. Four Halosarpheia spp., Lignincola laevis
and Nais inornata were examined to determine the placement of these genera
within the unitunicate ascomycetes. Two equally most parsimonious cladogram
s resulted from the analysis of the data. In a strict consensus of the two
cladograms the taxa sampled did not form a monophyletic group, and Halosarp
heia was shown to be polyphyletic. The marine Halosphaeriales formed a subc
lade with taxa from the Microascales. Data indicate that L. laevis and N. i
nornata may be congeneric, but other species of both genera require further
study.