A HIGHLY SENSITIVE, COMPACT RAMAN SYSTEM WITHOUT A SPECTROMETER FOR QUANTITATIVE-ANALYSIS OF BIOLOGICAL SAMPLES

We propose here a highly-sensitive, compact Raman system for quantitat ive analysis of biological samples. The system consists of a semicondu ctor laser, spherical integration type of cell-holder, Raman shift ban dpass filter, photodiode, multimeter and personal computer. It dose no t have a spectrometer. Therefore, one can not measure a Raman spectrum by use of the above system, but it is superb for estimating the inten sity of a particular Raman band. In other words, the system is very su itable for quantitative analysis of a particular sample. We applied th is system to determine the concentrations of glucose and acetone in ur ine containing glucose and/or acetone artificially. A Raman band at 11 30.5 cm(-1) due to glucose and that at 796.5 cm(-1) due to acetone wer e used for the above purpose. The correlation coefficient between the concentration and the Raman intensity and the detection limit were fou nd to be 0.992 and 31 mg/dl, respectively, for glucose. Those for acet one were calculated to be 0.991 and 8 mg/dl, respectively.