Dg. Moran et al., EXPRESSION OF GLYCOPROTEIN-PROCESSING ENZYMES IN ESCHERICHIA-COLI AS FUSION PROTEINS, World journal of microbiology & biotechnology, 13(3), 1997, pp. 265-267
A truncated Man(9)-mannosidase gene and a full-length beta-1,4-galacto
syltransferase gene were isolated from human kidney and placenta cDNAs
, respectively. Bath genes were cloned in plasmid pMAL-c2 to produce f
usions with maltose-binding protein. Fusion products were purified by
affinity chromatography. purified enzymes were assayed using pyridyl-a
mino labelled oligosaccharides as substrates and analysed by HPLC.