Vm. Martinez et al., PRODUCTION OF INDOLE-3-ACETIC-ACID BY SEVERAL WILD-TYPE STRAINS OF USTILAGO-MAYDIS, World journal of microbiology & biotechnology, 13(3), 1997, pp. 295-298
Indole-3-acetic acid (IAA) was identified and quantitated in spent med
ia from cultures of ten Ustilago maydis strains. IAA was identified by
thin-layer chromatography, high performance liquid chromatography (HP
LC) and u.v. spectroscopy, and was quantitated by HPLC. All strains pr
oduced IAA in a tryptophan (Trp)-supplemented minimal medium at levels
of 0.1 to 4.0 mu g IAA/ml of spent medium as assessed by HPLC The hig
hest levels of IAA were found in strains I2 and P2. The latter was als
o capable of producing IAA without addition of Trp to the medium.