H. Harmens et al., Does down-regulation of photosynthetic capacity by elevated CO2 depend on N supply in Dactylis glomerata?, PHYSL PLANT, 108(1), 2000, pp. 43-50
Dactytlis glomerata was grown hydroponically in a controlled environment at
ambient (360 mu l I (- 1)) or elevated (680 mu l I (- 1)) CO, and four con
centrations of nitrogen (0.15, 0.6, 1.5 and 6.0 mM NO3-), to test the hypot
hesis that reduction of photosynthetic capacity at elevated [CO2] is depend
ent on N availability and mediated by a build-up of non-structural carbohyd
rates. Photosynthetic capacity of the youngest fully expanded leaf (leaf 5,
2 days after full expansion) was reduced in CO2-enriched plants at low, bu
t not high N supply and so the stimulation of net photosynthesis by CO2 enh
ancement was less at low than at high N supply, CO2 enrichment resulted in
a decrease in ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) con
tent on a leaf area basis at 0.6 and 1.5 mM NO3- but not at 0.15 and 6.0 mM
NO3- and had no effect on the total N content of the leaf on an area basis
, However, decreases in Rubisco content could be primarily accounted for by
a decrease in total N content of leaves, independent of [CO2]. A doubling
of the Rubisco content by increasing the N supply beyond 0.6 mM had only a
marginal effect on the maximum carboxylation velocity in vivo, suggesting t
hat the fraction of inactive Rubisco increased with increasing N supply. Al
though CO2-enriched plants accumulated more non-structural carbohydrates in
the leaf, the reduction of photosynthetic capacity at low N supply was not
mediated simply by a build-up of carbohydrates. In D. glomerata, the photo
synthetic capacity was mainly determined by the total N content of the leaf
.