Coordinated diurnal regulation of low- and high-affinity nitrate transporters in tomato

Two new members of the NRT2 gene family encoding high-affinity NO3- transpo rters were isolated from tomato by heterologous screening. Both cDNAs, LeNR T2;1 and LeNRT2;2, are highly similar in the coding region, however, signif icant nucleotide differences were found in the 3'-untranslated region. High -stringent Southern analysis with gene-specific probes indicated that the t wo genes are linked in the genome and are most probably derived from a rece nt gene duplication. Expression of both genes was restricted to roots. Alth ough no difference in expression between both genes could be demonstrated b y Northern analysis, LeNRT2;1 and LeNRT2;2 were isolated 56 and 3 times, re spectively, from a cDNA library of root hairs, indicating a dominant expres sion of LeNRT2:1 in root hairs of tomato seedlings. Maximum transcript leve ls of LeNRT2 peaked 4h after NO3- induction and coincided with those of the putative low-affinity NO3- transporter gene, LeNRT1;2. In contrast, 4h aft er NO3- supply LeNRT1;1 transcription was maximally repressed, reflecting a n opposite regulation. As transcript levels of LeNRT2 also increased indepe ndently from NO3- induction, diurnal gene expression was monitored. Ail inv estigated genes, LeNRT2, LeNRT1;1, LeNRT1;2 and LeNR, showed the highest pe ak in transcript levels 4 h after the onset of the light period and a secon d peak at the end of the light period. Although expression of these genes w as highly coordinated at these times of the day, LeNRT1;1, LeNRT2;1 and LeN R showed additional peaks. This indicates that diurnal oscillation of these genes is most likely based on multiple factors, such as carbohydrate suppl y to the roots, feedback regulation from N assimilates and phytochrome-medi ated regulation.