Production and characterization of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-4-hydroxybutyrate) synthesized by Ralstonia eutropha in fed-batch cultures
La. Madden et al., Production and characterization of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-4-hydroxybutyrate) synthesized by Ralstonia eutropha in fed-batch cultures, POLYMER, 41(10), 2000, pp. 3499-3505
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-6hydroxybutyrate) [P(3HB-co-
3HV-co-4HB)] terpolymers were produced in fed-batch cultures of Ralstonia e
utropha (reclassified from Alcaligenes eutrophus) using glucose, propionic
acid and either 4-hydroxybutyric acid or gamma-butyrolactone as carbon sour
ces. The thermal and mechanical properties of terpolymers containing differ
ent proportions of the constituent monomers were characterized. Most of the
terpolymers possessed low melting temperatures and the majority possessed
crystallization half-times between 4 and 16 min, compared with 0.5 min for
poly(3-hydroxybutyrate) [P(3HB)]. For certain polymer compositions, contain
ing approximately 10 mol% of both 3-hydroxyvalerate (3HV) and 4-hydroxybuty
rate (4HB), the mechanical properties were superior to those of P(3HB-co-0-
30% 3HV) range of polymers and did not display any signs of deterioration o
ver a one month test period. A compression molded polymer film containing 7
8 mol% 3HB, 11 mol% 3HV and 11 mol% 4HB was found to possess an elongation
to break of 700% after one month, compared with 740% after molding, but ult
imately declined to 35% after 13 months. The aging process was found to dep
end upon the primary crystallization rate. (C) 2000 Elsevier Science Ltd. A
ll rights reserved.