Crystallization and preliminary X-ray diffraction analysis of Thermus thermophilus prolyl-tRNA synthetase

Authors
Yaremchuk, A Cusack, S Tukalo, M
Citation
A. Yaremchuk et al., Crystallization and preliminary X-ray diffraction analysis of Thermus thermophilus prolyl-tRNA synthetase, ACT CRYST D, 56, 2000, pp. 195-196
Citations number
7
Categorie Soggetti
Chemistry & Analysis
Journal title
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN journal
09074449 → ACNP
Volume
56
Year of publication
2000
Part
2
Pages
195 - 196
Database
ISI
SICI code
0907-4449(200002)56:<195:CAPXDA>2.0.ZU;2-P
Abstract
Prolyl-tRNA synthetase from Thermus thermophilus (ProRSTT) was purified to homogeneity using a five-step purification procedure and was crystallized u sing ethylene glycol as a precipitant. Crystals of ProRSTT belong to the sp ace group P2(1)2(1)2, with unit-cell parameters a = 132, b = 191, c = 125 A ngstrom, have two homodimers per asymmetric unit and diffract to 2.4 Angstr om resolution. A complete native data set to 2.43 Angstrom resolution has b een collected and a data set from ProRSTT in complex with proline has been collected to 2.9 Angstrom resolution.