Crystallization and preliminary X-ray studies of recombinant amylosucrase from Neisseria polysaccharea

Authors
Skov, LK Mirza, O Henriksen, A De Montalk, GP Remaud-Simeon, M Sarcabal, P Willemot, RM Monsan, P Gajhede, M
Citation
Lk. Skov et al., Crystallization and preliminary X-ray studies of recombinant amylosucrase from Neisseria polysaccharea, ACT CRYST D, 56, 2000, pp. 203-205
Citations number
11
Categorie Soggetti
Chemistry & Analysis
Journal title
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN journal
09074449 → ACNP
Volume
56
Year of publication
2000
Part
2
Pages
203 - 205
Database
ISI
SICI code
0907-4449(200002)56:<203:CAPXSO>2.0.ZU;2-X
Abstract
Recombinant amylosucrase from Neisseria polysaccharea was crystallized by t he vapour-diffusion procedure in the presence of polyethylene glycol 6000. The crystals belong to the orthorhombic space group P2(1)2(1)2, with unit-c ell parameters a = 95.7, b = 117.2, c = 62.1 Angstrom, and diffract to 1.6 Angstrom resolution. A p-chloromercuribenzene sulfonate (pcmbs) derivative has been identified and a selenomethionine-substituted protein has been pro duced and crystallized.