Gb. Kim et al., APPLICATION OF P450RGS (REPORTER GENE SYSTEM) AS A BIOINDICATOR OF SEDIMENT PAH CONTAMINATION IN THE VICINITY OF INCHEON HARBOR, KOREA, Biomarkers, 2(3), 1997, pp. 181-188
Sixty-seven sediment samples were collected from Kyeonggi Bay, Korea,
including the mouth of Han River Incheon Harbor, the Namdong industria
l complex, and the open sea. Collections were conducted in December, 1
995 and samples were maintained frozen (-20 degrees C) until analysis.
Dichloromethane extracts were analysed for their content of CYP1A1-in
ducing compounds with a P450RGS (reporter gene system) assay, and for
polycyclic aromatic hydrocarbons (PAHs). Sediment samples were also an
alysed for organic carbon (OC) content and grain size, to aid in evalu
ating the relationship between contamination and physical nature of th
e sediments. The responses of the P450RGS assay to the sediment extrac
ts were expressed as mu g of benzo[a]pyrene toxic equivalents per g dr
y weight (mu g g(-1) BaPTEQ), and these values correlated well (r(2)=0
.624) with total PAHs. BaPTEQ values were also highly correlated with
the OC content of the sediments. The determination of P450RGS BaPTEQ i
s a useful tool, because it is both a rapid and inexpensive means of a
ssessing the potential toxicity of organic compounds in environmental
sediment samples. These values represent an estimate of the levels of
compounds in the sediment that are potentially available to organisms
through chronic exposure to pore water or ingestion of benthic species
, We believe BaPTEQ values are more useful than tables of specific PAH
concentrations, if the purpose of the investigation is to either obta
in a rapid screening of an area or to develop some form of ecological
or human health risk assessment.