Vg. Nielsen et Ms. Baird, Extreme hemodilution in rabbits: An in vitro and in vivo thrombelastographic (R) analysis, ANESTH ANAL, 90(3), 2000, pp. 541-545
Citations number
19
Categorie Soggetti
Aneshtesia & Intensive Care","Medical Research Diagnosis & Treatment
Isovolemic hemodilution is used to decrease the incidence of blood transfus
ions. However, the effects of the degree of hemodilution and the fluid used
on hemostasis are controversial. We tested the hypothesis that hemodilutio
n and the fluid administered would adversely alter Thrombelastographic(R) (
Haemoscope, Skokie, IL) variables (reaction time, alpha angle and maximal a
mplitude). Conscious rabbits had blood sampled from ear arteries and dilute
d 0% or 75% in viiro with one of four solutions: 6% hetastarch in 0.9% NaCl
, 5% human albumin in 0.9% NaCl, or balanced electrolyte solutions containi
ng either 6% pentastarch or 6% hetastarch. Isoflurane-anesthetized rabbits
were randomly assigned to groups (n = 9 per group) that underwent in vivo i
sovolemic hemodilution (75% of estimated blood volume removed), with blood
replaced with one of the four solutions mentioned previously. In vitro hemo
dilution resulted in a significant (P < 0.05) decrease in hemostatic functi
on (increase in reaction time, decrease in a angle and maximal amplitude) t
hat was largest after hemodilution with albumin. However, although in vivo
hemodilution significantly (P < 0.05) decreased reaction time, increased th
e a angle, and decreased maximal amplitude, there were no significant fluid
-dependent effects. Implications: The effects of hemodilution and the fluid
used on Thrombelastographic(R) (Haemoscope, Skokie, IL) variables are mark
edly different between in vitro and in vivo hemodilution studies.