Crystallization and preliminary X-ray crystallographic analysis of alginate lyase Al-II from Sphingomonas species Al

Alginate lyase Al-II of Sphingomonas species Al was purified and crystalliz ed using the hanging drop vapor-diffusion method in 0.1 M Tris-HCl buffer c ontaining 43% saturated ammonium sulfate, 8% polyethylene glycol 4000 and 0 .2 M Li2SO4 at PH 8.5 and 20 degrees C. The crystals are tetragonal and bel ong to the space group P4(3)2(1)2 or P4(1)2(1)2 with unit cell dimensions o f a = b = 144.07 and c = 296.38 Angstrom. The diffraction data up to 2.8 An gstrom were collected by a synchrotron radiation source at SPring-8 in Japa n. (C) 2000 Elsevier Science B.V. All rights reserved.