Involvement of protein kinase A and a kinase anchoring protein in the progesterone-initiated human sperm acrosome reaction

The signal transduction pathways involved in the progesterone (P-4)-initiat ed mammalian sperm acrosome reaction (AR) are not fully understood. To inve stigate the role of the protein kinase A (PKA) pathway in the P-4-initiated AR, we probed this pathway by pretreating capacitated human sperm with rea gents designed to either inhibit PKA activation or disrupt PKA/A kinase anc horing protein (AKAP) interactions. Preincubation with the stearated (membr ane permeable) PKA inhibitor, PKI alpha 5-24 (S-PKI alpha 5-24), significan tly inhibited the P-4-initiated AR at 10 mu M as compared to stearated cont rol peptide. In contrast, preincubation with 100 mu M nonstearated PKI alph a 5-24 did not significantly inhibit versus solvent control. Preincubation with the PKA inhibitor Rp-8-Br-cAMP at 500 mu M and 150 mu M significantly inhibited the P-4-initiated AR versus 8-Br-cAMP and versus solvent. Preincu bation with the anchoring inhibitory peptide S-Ht31 significantly stimulate d the P-4-initiated AR at 10, 3, and 1 mu M versus inactive control peptide , The stimulation of the P-4-initiated AR by 3 mu M S-Ht31 was significantl y inhibited by the addition of 30 mu M S-PKI alpha 5-24 prior to the additi on of S-Ht31. Preincubation with S-PKI alpha 5-24 (30 mu M) partially inhib ited the ionomycin (50 mu M)-initiated AR. A role for PKA in the P-4-initia ted AR may exist both upstream and downstream of Ca2+ entry. Our studies pr esent the first evidence for the participation of PKA in the P-4-initiated AR and also suggest that AKAPs are involved in the PKA-mediated events.