Synthesis of the fatty sterol bound protein for a new sterol antibody

For the purpose of applying the particular antibodies as a new diagnostic p rocedure for atherosclerosis and related diseases. we successfully achieved the synthesis of the fatty sterol with a linker, then linked the target pr otein to this sterol. Synthesis was started from pregnenolone and achieved by the Grignard reaction with pentenyl magnesium bromide, regioselective ph oto-addition of thiolacetic acid toward the 25-double bond, esterification of 3-OH with linoleic anhydride, in situ conjunction of the cross-linker (M BS) to the thiol group after selective deprotection from its acetyl ester, and finally by the reaction with protein such as KLH or albumin through thi s linker. (C) 2000 Elsevier Science Ltd. All rights reserved.