Immune reconstitution after transplantation of autologous peripheral CD34(+) cells: analysis of predictive factors and comparison with unselected progenitor transplants

The recovery of lymphocyte count, CD4(+) and CD8(+) T-cell subsets, natural killer (NK) cells and CD19(+) B-cells was evaluated in a cohort of 15 pati ents receiving autologous CD34(+) peripheral blood progenitor cells (PBPCs; group A) for haematological malignancies and in 20 patients transplanted w ith autologous unselected PBPCs (group B). Lymphocyte count recovered in bo th patient cohorts, being significantly lower in group A than in group B 1 (P = 0.008) and 2 months (P = 0.0035) after progenitor cell infusion. The r epopulation of CD3(+) T-cells occurred more rapidly in group B than in grou p A (P = 0.034 on week): CD19(+) B-lymphocytes did not return to reference ranges in either group of patients. The count of CD4(+) T-lymphocytes remai ned <200/mu l during the study period in patients transplanted with CD34(+) PBPCs, significantly lower than group B levels (P = 0.034 and P = 0.021 on weeks 4 and 8 respectively), CD8(+) T-cells increased rapidly in both grou ps; thus, the CD4 to CD8 ratio was severely reduced. CD4(+) and CD8(+) T-ce lls displayed an activated phenotype in both groups of patients, co-express ing the HLA-DR antigen throughout the study period. NK cells followed a sim ilar repopulation kinetics in both study groups, although their expansion w as greater in group B than in group A (P = 0.014 on week 4), In the CD34(+) group, post-transplant administration of granulocyte colony-stimulating fa ctor predicted a faster lymphocyte recovery in multivariate analysis (P = 0 .025); interestingly, the amount of passively transferred lymphocytes corre lated inversely with time to achieve a lymphocyte count >0.5 x 10(9)/l (r = -0.63, P = 0.01). Further investigations are necessary to characterize T-c ell competence after transplantation of CD34(+) PBPCs.