Demonstration of mRNA for five species of cytochrome P450 in human bone marrow, bone marrow-derived macrophages and human haemopoietic cell lines

The expression of mRNA for five cytochrome P450s (CYP1A1, 2A6/7, 2D6, 2E1 a nd 3A4) was studied in human bone marrow, bone-marrow-derived macrophages a nd blood monocyte-derived macrophages. Reverse transcriptase polymerase cha in reaction (RT-PCR) detected expression of all Eve CYPs in each of these c ell populations, All five CYPs were also expressed in the haemopoietic cell lines HL-60 and HEL and in Epstein-Barr virus (EBV)-transformed B-lymphobl astoid cell lines. The data suggest that bone marrow macrophages and probab ly other bone marrow cell types are capable of metabolizing xenobiotics. Th is metabolic potential may play a role in the bone marrow damage induced by some drugs and chemicals.