An experimental study on systemic inflammatory response syndrome induced by subeschar tissue fluid

Objective: to investigate the biological activity of subeschar tissue fluid (STF) and its probable mechanism in the genesis of systemic inflammatory r esponse syndrome(SIRS). Methods: the changes of heart rate (HR), respirator y rate (RR), white blood cell count (WBCC) as well as the major organ funct ion were observed in the animals injected with STF. The inflammatory mediat ors TNF-alpha and IL-1 in the supernatants of macrophages cultured with STF were assayed. Results: the HR, RR and WBCC were elevated in animals after injection with STF. STF showed a deleterious effect on function and structu re of the major visceral organs. Macrophages were activated to produce exce ssive TNF-alpha and IL-1. Conclusion: the findings suggest that STF may be one of the inducing factors involved in the genesis of SIRS and the develop ment of MODS in the early postburn stage. (C) 2000 Elsevier Science Ltd and ISBI. All rights reserved.