In this opinion paper, we offer our perspective on our bioremediation
research along with the methods to assess its effectiveness as a safe
and beneficial technology to remediate selected soil sites. The isolat
ion and characterization of bacterial isolates from chemically contami
nated soils, their survival and catabolic activity in contaminated soi
l, toxicity testing in chemically contaminated soils, molecular-based
methods of detection such as the polymerase chain reaction (PCR) and D
NA probing are discussed. By using numerous conventional microbiologic
al, chemical techniques and molecular based methods, bioremediation ca
n be studied in a comprehensive manner and the technology transferred
to the commercial sector.