Sputum elastase in steady-state bronchiectasis

Study objectives: To study. the correlations between sputum elastase output with clinical and sputum inflammatory and microbial factors in steady-stat e bronchiectasis. Design: Prospective recruitment of patients with bronchiectasis (17 women; 48.5 +/- 16.5 years old; FEV1/FVC, 1.3 +/- 0.6/2.1 +/- 0.9) for assessment of 24-h sputum output of elastase, bacteria, leukocytes, interleukin (IL)-1 beta, IL-8, tumor necrosis factor-alpha, and leukotriene B-4, Clinical var iables assessed concomitantly included 24-h sputum volume, lung spirometry, number of lung lobes affected by bronchiectasis, and exacerbation frequenc y. Setting: Consecutive recruitment of outpatients (n = 30) in steady-state br onchiectasis. Measurements and results: Twenty-four-hour sputum elastase output correlate d with 24-h sputum volume (r = 0.79, p = 0.0001); number of bronchiectatic lung lobes (r = 0.54, p = 0.0026); percent predicted FEV1 (r = -0.48, p = 0 .0068); percent predicted FVC (r = -0.49, p = 0.001); and leukocyte output (r = 0.75, p = 0.0001). There was no correlation between the sputum output of bacteria with either inflammatory or enzymatic factors (p > 0.05). Conclusions: Our data highlight the importance of elastase and the possibil ity of independent roles for enzymatic, inflammatory, and microbial compone nts in the pathogenesis of bronchiectasis. Further research on novel therap y targeting each of these components should be pursued.