Modelisation of leukocyte adhesion on a fibrinogen coated surface in static conditions

The adhesion of polymorphonuclear leukocytes (PMNs) on the vascular endothe lium is a complex process that occurs during biological and pathological ev ents and involves a large family of molecules. This phenomenom could be app roached by a modelisation study of the adhesion of PMNs on a biological sub strate, fibrinogen. Two different physiological conditions were tested such as the activated state of PMNs with a synthetic pro-inflammatory activator (N-Formyl-Methionyl-Leucyl-Phenylalanin, FMLP). The activated state of PMN s was both quantified by Row cytometry and controlled by fluorescence micro scopy. The results suggest that quiescent PMNs deposit in accordance with the ball istic deposition model. The preliminary results obtained with FMLP-stimulat ed PMNs show a different deposit process compared to quiescent PMNs but do not allow to determine exactly a deposition model.