Spontaneous high systemic oxygen delivery increases survival rate in awakesheep during sustained endotoxemia

Objective: To study the natural evolution of systemic oxygen delivery ((D o ver dot O-2) and oxygen consumption ((V) over dot O-2) in sheep infused wit h low or high doses of endotoxin, Design: Prospective, controlled experimental study. Setting: Animal research laboratory at a medical university. Subjects: Twenty-nine chronically instrumented awake sheep (25-35 kg), Interventions: Awake animals were continuously infused with saline (n = 8) or two doses of Escherichia coli endotoxin (20 or 40 ng/kg/min; n = 21) for 72 hrs, No attempt was made to increase (D) over dot O-2, but respiratory failure was treated by mechanical ventilation and metabolic acidosis was co rrected. Measurements and Main Results: The mortality rate was 25% in the group infu sed with the low dose and 89% in the group infused with the high dose of en dotoxin, During the first 12 hrs of endotoxemia, both surviving (S group; n = 10) and nonsurviving (NS group; n = 11) sheep developed similar pulmonar y hypertension, left ventricular failure, and hypotension with low systemic vascular resistance. However, S sheep had less interstitial lung edema (pu lmonary lymph protein clearance at 8 hrs was 13 +/- 3 mL/hr vs. 27 +/- 6 mL /hr in the NS group and 4 +/- 1 mL/hr in the control group). During this ea rly phase of endotoxemia, (D) over dot O-2, (V) over dot O-2, and oxygen ex traction ratio did not change significantly in any group. After this phase, animals that ultimately survived had a persistent hyperdynamic syndrome wi th high cardiac output and hypotension. In this group, the (D) over dot O-2 increase was greater than the (D) over dot O-2 measured in controls and re mained steady up to 48 hrs after the start of the endotoxin infusion. Becau se systemic (V) over dot O-2 did not change significantly, oxygen extractio n ratio decreased progressively to values less than those measured in contr ols. In contrast, animals that ultimately died had a hypotensive and normok inetic syndrome associated with pulmonary hypertension, persistent depresse d left ventricular function, hypothermia, and a progressive deterioration o f gas exchange, Systemic (D) over dot O-2 was not significantly different f rom that in the control group. In contrast, ire, decreased progressively to values significantly lower than those measured in controls and remained lo w until death, Conclusions: Our results indicate that in the absence of treatment such as fluid challenge or inotropic drugs in sheep infused with endotoxin, the occ urrence of spontaneous hyperdynamic syndrome and high (D) over dot O-2 impr oves the survival rate.