Cyclooxygenase-independent inhibition of smooth muscle cell mitogenesis byibuprofen

The aryl-propionic acid derivative, ketoprofen, has been shown to inhibit f ibroblast growth by a cylooxygenase-dependent mechanism [Sanchez, T., Moren o, J.J., 1999. S(+) enantiomer inhibits prostaglandin production and cell g rowth in 3T6 fibroblast cultures. Eur. J. Pharmacol. 370, 63-67]. The prese nt study demonstrates that ibuprofen, another aryl-propionic acid derivativ e, inhibited platelet-derived growth factor-BB (20 ng/ml)-induced mitogenes is of cultured bovine coronary artery smooth muscle cells in a stereo-indep endent manner. In addition, pretreatment of the cells with indomethacin (3 mu M) did not affect the inhibitory effects of ibuprofen enantiomers on smo oth muscle cell mitogenesis. Thus, aryl-propionic acid-type cyclooxygenase inhibitors can inhibit cell proliferation by both, cyclooxygenase-dependent and -independent ways. (C) 2000 Elsevier Science B.V. All rights reserved.