Jl. Newton et al., The human trefoil peptide, TFF1, is present in different molecular forms that are intimately associated with mucus in normal stomach, GUT, 46(3), 2000, pp. 312-320
Background-TFF1 is a 6.5 kDa secreted protein that is expressed predominant
ly in normal gastric mucosa. It is coexpressed with mucins and it can form
dimers via a free carboxy terminal cysteine residue.
Aims-To investigate the molecular forms of TFF1 that are present in normal
human stomach and the association of the different molecular forms with muc
us.
Subjects-All subjects had macroscopically normal stomachs at gastroscopy. N
one had a significant past medical history.
Methods TFF1 gastric mucosa and adherent mucus by western transfer analysis
after electrophoresis on reducing and non-reducing polyacrylamide gels. In
some instances, proteins were fractionated by caesium chloride density gra
dient centrifugation prior to detection of TFF1. The location of TFF1 in ga
stric mucosa with an intact adherent mucus layer was assessed by immunohist
ochemistry.
Results-Three different molecular forms of TFF1 were detected: TFF1 monomer
, TFF1 dimer, and a TFF1 complex with an apparent molecular mass of about 2
5 kDa. TFF1 was present at higher concentrations than realised previously.
The TFF1 complex was present in the adherent mucus gel layer but while its
interaction with mucin was destabilised by caesium chloride, the interactio
n between mucin and the TFF1 dimer was resistant to caesium chloride.
Conclusions-Most of TFF1 in normal human gastric mucosa is present in a com
plex that is stabilised by a disulphide bond. TFF1 is intimately associated
with mucus. The high concentration, colocalisation, and binding of TFF1 to
gastric mucus strongly implicate TFF1 in gastric mucus function.