KC production in the cornea in response to Pseudomonas aeruginosa challenge

Citation
N. Cole et al., KC production in the cornea in response to Pseudomonas aeruginosa challenge, IMM CELL B, 78(1), 2000, pp. 1-4
Citations number
10
Categorie Soggetti
Immunology
Journal title
IMMUNOLOGY AND CELL BIOLOGY
ISSN journal
08189641 → ACNP
Volume
78
Issue
1
Year of publication
2000
Pages
1 - 4
Database
ISI
SICI code
0818-9641(200002)78:1<1:KPITCI>2.0.ZU;2-Z
Abstract
Pseudomonas aeruginosa can cause ulcerative bacterial keratitis. A feature of keratitis is the rapid infiltration of the avascular corneal stroma by n eutrophils. KC is a potent neutrophil chemokine. The present study used a m ouse model of ocular infection to assess the relationship between KC and in flammation in the cornea in response to challenge with a strain of P. aerug inosa causing keratitis. Low levels of KC mRNA acid protein were detected b y in situ hybridization and ELISA, respectively, in unchallenged corneas. D ramatically increased numbers of KC mRNA(+) cells were present in P. aerugi nosa strain 6294-challenged corneas. Expression of KC mRNA was found to be up-regulated in the corneal epithelium in response to wounding alone. The K C mRNA(+) cells were located in the epithelium and corresponding to infiltr ating neutrophils cells in the stroma. Quantification of KC protein at diff erent time points showed peak levels at 8 h of bacterial challenge. These r esults suggest that KC may be involved with the regulation of leucocyte inf iltration early during bacterial keratitis.