Functional scFv antibody sequences against the organophosphorus pesticide chlorpyrifos

Authors
Alcocer, MJC Doyen, C Lee, HA Morgan, MRA
Citation
Mjc. Alcocer et al., Functional scFv antibody sequences against the organophosphorus pesticide chlorpyrifos, J AGR FOOD, 48(2), 2000, pp. 335-337
Citations number
16
Categorie Soggetti
Agricultural Chemistry","Chemistry & Analysis
Journal title
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN journal
00218561 → ACNP
Volume
48
Issue
2
Year of publication
2000
Pages
335 - 337
Database
ISI
SICI code
0021-8561(200002)48:2<335:FSASAT>2.0.ZU;2-8
Abstract
Two functional single-chain Fv (scFv) antibodies that recognize specificall y the widely used organophosphorus pesticide chlorpyrifos-ethyl were derive d from two murine hybridoma cell lines. It is shown that the functional scF vs could be isolated without any rounds of selection, with a success rate d ependent on the efficiency of amplification of the functional light chain g ene family by the specific primers. Besides four new functional immunoglobu lin variable gene sequences, the isolation of a new pseudogene is reported.