Adsorption of human IgG on Cu2+-immobilized cellulose affinity membrane: Preliminary study

Immobilized metal ion affinity chromatography (IMAC) is widely used. Transi tion metal ions have a high affinity to some peptide sequences. We have stu died the selective adsorption of human IgG from a mixture of albumin, gamma -globulin, fibrinogen, and IgG onto Cu2+ ion-immobilized cellulose membrane . Although Cu2+ ligand is selective to IgG, in general gamma-globulins also are adsorbed. The simplicity and lower cost of Cu2+ ion-immobilized cellul ose membranes may be useful for removing IgG from blood. (C) 2000 John Wile y & Sons, Inc.