Analysis of barbiturates by micro-high-performance liquid chromatography with post-column photochemical derivatization

Authors
Garcia-Borregon, PF Lores, M Cela, R
Citation
Pf. Garcia-borregon et al., Analysis of barbiturates by micro-high-performance liquid chromatography with post-column photochemical derivatization, J CHROMAT A, 870(1-2), 2000, pp. 39-44
Citations number
16
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF CHROMATOGRAPHY A → ACNP
Volume
870
Issue
1-2
Year of publication
2000
Pages
39 - 44
Database
ISI
SICI code
Abstract
This study attempts to combine the advantages of microcolumn high-performan ce liquid chromatography (mu-HPLC) with those of post-column photochemical derivatization in barbiturate analysis. Some barbiturates are photochemical ly unstable, leading to photoproducts that show maximum absorption at 270 n m and not the typical one at approximate to 220 nm. For this purpose, a lab oratory-built photoreactor has been developed to work with mu-HPLC instrume nts. Its performance is satisfactory in the forensic analysis of barbiturat es. (C) 2000 Elsevier Science B.V. All rights reserved.