Validation of the removal of acetylsalicylic acid - Recovery and determination of residues on various surfaces by high performance liquid chromatographic

The validation of a procedure to clean glass, vinyl and stainless steel sur faces that have been exposed to acetylsalicylic acid during its manufacture is described. The cleaning procedure using two cotton swabs moistened with the mobile phase was validated using a wipe-test and a high-performance Li quid chromatography (HPLC) method developed to determine low quantities of the acid. The HPLC method involves an octadecylsilane column at 55 degrees C, a mixture of water-acetonitrile-orthophosphoric acid (779:220:1, v/v) as mobile phase and detection at 226 nm. Recoveries of 86%, 90% and 94% were obtained from vinyl, glass and stainless steel plates respectively. The val idation gave acceptable levels of sensitivity, recovery, precision and line arity. (C) 2000 Elsevier Science B.V. All rights reserved.